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KMID : 0385520150280020117
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Analytical Science & Technology
2015 Volume.28 No. 2 p.117 ~ p.124
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Establishment and application of a qualitative real-time polymerase chain reaction method for detecting genetically modified papaya line 55-1 in papaya products
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Kwon Yu-Jihn
Chung So-Young
Cho Kyung-Chul
Park Ji-Eun
Koo Eun-Joo
Seo Dong-Hyuk
Kim Eu-Gene
Whang Je-hyun
Park Seong-Soo
Choi Sun-Ok
Lim Chul-Ju
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Abstract
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Genetically modified (GM) papaya line 55-1, which is resistant to PRSV infection, has been marketed globally. Prompt and sensitive protocols for specific detections are essential for the traceability of this line. Here, an event- and construct-specific real-time polymerase chain reaction (RT-PCR) method was established to detect 55-1. Qualitative detection was possible for fresh papaya fruit up to dilutions of 0.005% and 0.01% for the homozygous SunUp and heterozygous Rainbow cultivars, respectively, in non-GM papaya. The method was applied in the qualitative detection of 55-1 in eight types of commercially processed papaya products. Additionally, papaya products were monitored to distinguish GM papaya using the P35S and T-nos RT-PCR detection methods. As expected, detection capacity was improved via modified sample preparation and the established RT-PCR detection method. Taking these results together, it can be suggested that a suitable method for the extraction and purification of DNA from processed papaya products was established for the detection of GM papaya.
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KEYWORD
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genetically modified, real-time polymerase chain reaction, papaya, 55-1, monitoring,
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